Reveal the regulation mechanisms of stem cell differentiation timing
June 14, Cell Stem Cell journal reported the latest progress of the timing of regulatory mechanisms of stem cell differentiation. The timing of cell differentiation are tightly regulated. Normal development and stem cell differentiation is critical. However, the mechanism of this regulation is not at all clear.
This study reveals the molecular mechanism of the timing of the decision mouse embryonic stem cells (ESC) differentiation. Protein kinase A (PKA) is activated to adjust the timing of differentiation, and accelerate the mesoderm and endoderm cells. Pluripotent markers, early disappearance of the ESC differentiation are interrelated.
PKA activation increases G9a, an H3K9 methyl transferase expression. Is accompanied by H3K9 methylation and Oct3 / 4 and Nanog gene promoter sites of DNA methylation. Knockout the G9a be completely inhibited by the PKA-induced differentiation processes and epigenetic modifications. Moreover, G9a knock In addition to the mice in the embryo 7.5 days showing extended Oct3 / 4 and Nanog gene expression, and developmental delay.
In conclusion, this study confirmed the regulation of of pedigree differentiation opportunity molecular machines linked signaling pathways and epigenetic modification of the operation
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